hPL ELISA Kit

CAT#: EA101020

hPL ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0.04 - 20 mg/ml
• Sample Type: Serum
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human hPL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The hPL ELISA is an enzyme immunoassay for measurement of human Placental Lactogen (hPL) in serum. The ORIGENE hPL ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the hPL molecule. An aliquot of specimen sample containing endogenous hPL is incubated in the coated well with enzyme conjugate, which is an anti- hPL antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of hPL in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of hPL in the specimen sample.