CEA ELISA Kit

CAT#: EA101036

CEA ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0 - 100 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 50 ul
• Specificity: Natural and recombinant human CEA
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The CEA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the CEA molecule. An aliquot of specimen sample containing endogenous CEA is incubated in the coated well with enzyme conjugate, which is an anti- CEA antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CEA in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CEA in the specimen sample.