HSV-1 IgG ELISA Kit

CAT#: EA101071

HSV-1 IgG ELISA Kit


USD 418.00

3 Weeks*

Size
    • 1 kit

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Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Sample Type Serum, plasma
Sample Volume 10 ul
Specificity Natural and recombinant human HSV-1 IgG
Reactivities Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The Herpes Simplex Virus Type 1 IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with recombinant gG1 protein of HSV-1. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Herpes Simplex Virus Type 1-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Herpes Simplex Virus Type 1-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.