Tetanus toxin IgG ELISA Kit

CAT#: EA101086

Tetanus toxin IgG ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human Tetanus toxin IgG
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The Tetanus toxin IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Tetanus toxoid antigen. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Tetanus toxin-specific antibodies of high specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of high results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Tetanus toxin-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.