PSA Total ELISA Kit

CAT#: EA101093

PSA Total ELISA Kit


USD 352.00

3 Weeks*

Size
    • 1 kit

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Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 0 - 25 ng/mL
Sample Type Cell culture supernatant, serum, plasma
Sample Volume 25 ul
Specificity Natural and recombinant human PSA Total
Reactivities Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background This assay is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with an antibody, directed towards an epitope of an antigen molecule (PSA). An aliquot of specimen sample serum is incubated in the coated well with enzyme conjugated second antibody (E-Ab), directed towards a different region of the antigen molecule. After incubation the unbound E-Ab is washed off. The amount of bound E-Ab is proportional to the concentration of antigen in the sample. After adding the substrate solution, the intensity of color developed is proportional to the antigen concentration in the sample. The measured ODs of the standards are used to construct a calibration curve against which the unknown samples are calculated.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.