CYFRA 21-1 ELISA Kit

CAT#: EA101116

CYFRA 21-1 ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0.15 – 50 ng/mL
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human CYFRA 21-1
• Sensitivity: 0.15 ng/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The CYFRA 21 1 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a mouse monoclonal antibody directed towards a unique antigenic site of the CYFRA21 1 molecule. An aliquot of sample containing endogenous CYFRA21 1 is incubated in the coated well with enzyme conjugate, which is an anti- CYFRA21 1 monoclonal antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CYFRA21 1 in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of CYFRA21 1 in the sample.