Salivary DHEA ELISA Kit

CAT#: EA101133

Salivary DHEA ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0 - 1440 pg/mL
• Sample Type: Cell culture supernatant, serum, plasma, saliva
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human Salivary DHEA
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The Salivary DHEA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site of the DHEA molecule. Endogenous DHEA of a sample competes with a DHEA-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of DHEA in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of DHEA in the sample.