Salivary Estrone ELISA Kit

CAT#: EA101138

Salivary Estrone ELISA Kit


USD 627.00

3 Weeks*

Size
    • 1 kit

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Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Competitive ELISA
Assay Length 3 hour
Signal Colorimetric
Curve Range 0 - 300 pg/mL
Sample Type Cell culture supernatant, serum, plasma, saliva
Sample Volume 10 ul
Specificity Natural and recombinant human Salivary Estrone
Reactivities Human
Interference No significant interference observed with available related molecules.
Components See the product information for more details
Background The Salivary Estrone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with streptavidin and biotinylated Estrone molecules. Endogenous Estrone of a specimen sample competes with this immobilized Estrone for binding sites of a HRP-conjugated sheep antibody directed towards an antigenic site of estrone. After incubation the unbound antibody conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estrone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Estrone in the specimen sample.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.