Salivary Estrone ELISA Kit

CAT#: EA101138

Salivary Estrone ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Competitive ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Curve Range: 0 - 300 pg/mL
• Sample Type: Cell culture supernatant, serum, plasma, saliva
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human Salivary Estrone
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The Salivary Estrone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with streptavidin and biotinylated Estrone molecules. Endogenous Estrone of a specimen sample competes with this immobilized Estrone for binding sites of a HRP-conjugated sheep antibody directed towards an antigenic site of estrone. After incubation the unbound antibody conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estrone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Estrone in the specimen sample.