Human IL-12 HS ELISA Kit, 1 x 48-well (pre-coated)

CAT#: EA101223

Human IL-12 HS ELISA Kit, 1 x 48-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 1 x 48-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4h00min
• Signal: Colorimetric
• Curve Range: 0.78-25 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human IL-12. Specific to p70 heterodimer.
• Sensitivity: 0.75 pg/ml
• Cross Reactivity: No cross reactivity with other human cytokines
• Interference: No significant interference observed with available related molecules.
• Components: High Sensitivity ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]
• Gene Symbol: IL12B
• Standard Curve:
  
  Representative Standard Curve