Human MICA ELISA Kit, 2 x 96-well (pre-coated)

CAT#: EA101255

Human MICA ELISA Kit, 2 x 96-well (pre-coated)

Specifications

Product Data

• Format: 2 x 96-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3h50min
• Signal: Colorimetric
• Curve Range: 156-5000 pg/ml
• Sample Type: Supernatant, Buffered Solutions. Not suitable for use with serum and plasma.
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human MICA
• Sensitivity: 123 pg/ml
• Cross Reactivity: No cross reactivity with IL-7, IL-12p40, IL-2R, Trail, CD31, IL-13Ra2, IL-5, gp130, TNFa.
• Interference: No significant interference observed with available related molecules.
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]
• Gene Symbol: MICA
• Standard Curve:
  
  Representative Standard Curve