Human IL-10 ELISA Set, 1 x 96-well

CAT#: EA101297

Human IL-10 ELISA Set, 1 x 96-well

Specifications

Product Data

• Format: 1 x 96-well
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: From sample to end 2h45min
• Signal: Colorimetric
• Curve Range: 12.5-400 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human IL-10
• Sensitivity: 5 pg/ml
• Cross Reactivity: No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10. No cross reactivity with viral IL-10.
• Components: ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
• Background: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2011]
• Gene Symbol: IL10