Human TNF alpha ELISA Set, 5 x 96-well

CAT#: EA101313

Human TNF alpha ELISA Set, 5 x 96-well

Specifications

Product Data

• Format: 5 x 96-well
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: From sample to end, 2 h to 4 h
• Signal: Colorimetric
• Curve Range: 25-800 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human TNF-a
• Sensitivity: 10 pg/ml
• Cross Reactivity: No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
• Components: ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
• Background: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
• Gene Symbol: TNF