Human IL-10 ELISpot Set, 1 x 96-well (plate not included)

CAT#: EA101463

Human IL-10 ELISpot Set, 1 x 96-well (plate not included)

Specifications

Product Data

• Format: 1 x 96-well (plate not included)
• Assay Type: Enzyme-Linked ImmunoSpot (ELISpot)
• Assay Length: 3h after cell stimulation
• Signal: Colorimetric
• Specificity: Recognizes natural human IL-10
• Cross Reactivity: No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
• Components: ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB, blocking reagent.
• Background: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2011]
• Gene Symbol: IL10