Murine TNF alpha ELISA Kit, 2 x 96-well (pre-coated)

CAT#: EA101695

Murine TNF alpha ELISA Kit, 2 x 96-well (pre-coated)

Specifications

Product Data

• Format: 2 x 96-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3h45min
• Signal: Colorimetric
• Curve Range: 31.25-1000 pg/ml
• Sample Type: Serum, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant murine TNF-a
• Sensitivity: 25 pg/ml
• Cross Reactivity: No cross reactivity with other murine cytokines
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
• Gene Symbol: Tnf
• Standard Curve:
  
  Representative Standard Curve