Human TNF alpha ELISA Kit, 2 x 96-well (pre-coated)

CAT#: EA102165

Human TNF alpha ELISA Kit, 2 x 96-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 2 x 96-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3h40min
• Signal: Colorimetric
• Curve Range: 25-800 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human TNF-a
• Sensitivity: 10 pg/ml
• Cross Reactivity: No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
• Gene Symbol: TNF
• Standard Curve:
  
  Representative Standard Curve