Human CD25 ELISA Kit, 1 x 48-well (pre-coated)

CAT#: EA102166

Human CD25 ELISA Kit, 1 x 48-well (pre-coated)

Product Images

Representative Standard Curve

Specifications

Product Data

• Format: 1 x 48-well (pre-coated)
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3h40min
• Signal: Colorimetric
• Curve Range: 68.75-2220 pg/ml
• Sample Type: Serum, Plasma, Cell culture supernatant
• Sample Volume: 100 µl
• Specificity: Recognizes both natural and recombinant human soluble IL-2R
• Sensitivity: 8 pg/ml
• Cross Reactivity: No cross reactivity with other human soluble receptors
• Components: ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
• Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. [provided by RefSeq, Nov 2009]
• Gene Symbol: IL2RA
• Standard Curve:
  
  Representative Standard Curve