Human IGF1 ELISA KIT (1 x 96 wells)

CAT#: EA102183

For quantitative detection of human IGF-1 in cell culture supernates, serum and plasma (heparin, EDTA).


USD 470.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human IGF-1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Specificity This kit is used for quantitative detection of Human IGF-1
Sensitivity <50pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with IGF-2.
Components
  • 96-well plate precoated with anti- human IGF-1 antibody | 1
  • Lyophilized recombinant human IGF-1 standard | 10ng/tube×2
  • Biotinylated anti- human IGF-1 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Gene Symbol IGF1
Standard Curve
Human IGF-1 ELISA Kit Standard Curve

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.