Human MMP14 ELISA KIT (1 x 96 wells)

CAT#: EA102240

For quantitative detection of human MMP-14 in cell culture supernates, serum and plasma(heparin).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for human MMP-14
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of human MMP-14
• Sensitivity: <15pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human MMP-14 antibody | 1
  • Lyophilized recombinant human MMP-14 standard | 10ng/tube×2
  • Biotinylated anti- human MMP-14 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
• Gene Symbol: MMP14
• Standard Curve:
  
  Human MMP-14 ELISA Kit standard curve