Mouse Cd44 ELISA KIT (1 x 96 wells)

CAT#: EA102297

For quantitative detection of mouse CD44 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).


USD 470.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for mouse CD44
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 312pg/ml-20000pg/ml
Specificity This kit is used for quantitative detection of mouse CD44
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- Mouse CD44 antibody | 1
  • Lyophilized recombinant mouse CD44 standard | 20ng/tube×2
  • Biotinylated anti- mouse CD44 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Gene Symbol Cd44
Standard Curve
Mouse CD44 ELISA Kit standard curve

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.