Human Angiotensinogen (AGT) ELISA KIT (1 x 96 wells)

CAT#: EA102330

For Quantitative Detection of human AGT in cell culture supernates, serum and plasma (EDTA).


USD 500.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human AGT
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Specificity This kit is used for quantitative detection of Human AGT
Sensitivity <10pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human AGT antibody | 1
  • Lyophilized recombinant human AGT standard | 10ng/tube×2
  • Biotinylated anti- human AGT antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background AGT(ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
Gene Symbol AGT
Standard Curve
Human AGT ELISA Kit standard curve

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.