Mouse Afp ELISA KIT (1 x 96 wells)

CAT#: EA102474

For quantitative detection of mouse AFP in cell culture supernates, serum and plasma (heparin, EDTA).


USD 470.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse AFP
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Specificity This kit is used for quantitative detection of Mouse AFP
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- mouse AFP antibody | 1
  • Lyophilized recombinant mouse AFP standard | 10ng/tube×2
  • Biotinylated anti- mouse AFP antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
Gene Symbol Afp
Standard Curve
Mouse AFP ELISA Kit Standard Curve

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.