Rat Pcsk9 ELISA KIT (1 x 96 wells)

CAT#: EA102502

For quantitative detection of rat PCSK9 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat PCSK9
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Specificity: This kit is used for quantitative detection of Rat PCSK9
• Sensitivity: <10pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat PCSK9 antibody | 1
  • Lyophilized recombinant rat PCSK9 standard | 4ng/tubex2
  • Biotinylated anti- rat PCSK9 antibody | 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: PCSK9 is the 9th member of the proprotein convertase family of proteins that activate other proteins.PCSK9 has medical importance because it acts in lipoprotein homeostasis. PCSK9 is ubiquitously expressed in many tissues and cell types. PCSK9 binds to the receptor for low-density lipoprotein particles (LDL), which typically transport 3,000 to 6,000 fat molecules (including cholesterol) per particle, within extracellular fluid. The LDL receptor (LDLR), on liver and other cell membranes, binds and initiates ingestion of LDL-particles from extracellular fluid into cells, thus reducing LDL particle concentrations.
• Gene Symbol: Pcsk9
• Standard Curve:
  
  Rat PCSK9 ELISA Kit standard curve