Rat Mag ELISA KIT (1 x 96 wells)

CAT#: EA102739

For quantitative detection of rat MAG in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat MAG
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 78pg/ml-5000pg/ml
• Specificity: This kit is used for quantitative detection of Rat MAG
• Sensitivity: <50pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat MAG antibody | 1
  • Lyophilized recombinant rat MAG standard | 10ng/tube×2
  • Biotinylated anti- rat MAG antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor(NgR). This gene is mapped to 1q21. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
• Gene Symbol: Mag
• Standard Curve:
  
  Rat MAG ELISA Kit Standard Curve