Rat Dner ELISA KIT (1 x 96 wells)

CAT#: EA102759

For quantitative detection of rat DNER in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat DNER
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20000pg/ml
• Specificity: This kit is used for quantitative detection of Rat DNER
• Sensitivity: <50pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat DNER antibody | 1
  • Lyophilized recombinant rat DNER standard | 20ng/tube×2
  • Biotinylated anti- rat DNER antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 9q35. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.
• Gene Symbol: Dner
• Standard Curve:
  
  Rat DNER ELISA Kit Standard Curve