Rat Chrd ELISA KIT (1 x 96 wells)

CAT#: EA102763

For quantitative detection of rat Chordin in cell culture supernates, serum and plasma (heparin, EDTA).


USD 470.00

3 Weeks*

Size
    • 1 x 96 wells

Product Images

Other products for "Chrd"

Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat Chordin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Specificity This kit is used for quantitative detection of Rat Chordin
Sensitivity <50pg/ml
Reactivities Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- rat Chordin antibody | 1
  • Lyophilized recombinant rat Chordin standard | 10ng/tube×2
  • Biotinylated anti- rat Chordin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
Gene Symbol Chrd
Standard Curve
Rat Chordin ELISA Kit Standard Curve

Documents

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.