Rat Cdh4 ELISA KIT (1 x 96 wells)

CAT#: EA102772

For quantitative detection of rat Cadherin-4/R-Cadherin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat Cadherin-4/R-Cadherin
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of Rat Cadherin-4/R-Cadherin
• Sensitivity: <50pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat Cadherin-4/R-Cadherin antibody | 1
  • Lyophilized recombinant rat Cadherin-4/R-Cadherin standard | 10ng/tube×2
  • Biotinylated anti- rat Cadherin-4/R-Cadherin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 3q43. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Gene Symbol: Cdh4
• Standard Curve:
  
  Rat Cadherin-4/R-Cadherin ELISA Kit Standard Curve