Rat Flt3lg ELISA KIT (1 x 96 wells)

CAT#: EA102778

For quantitative detection of rat Flt-3 ligand in cell culture supernates and serum.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat Flt-3 ligand
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Specificity: This kit is used for quantitative detection of Rat Flt-3 ligand
• Sensitivity: <10pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat Flt-3 ligand antibody | 1
  • Lyophilized recombinant rat Flt-3 ligand standard | 10ng/tube×2
  • Biotinylated anti- rat Flt-3 ligand antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.
• Gene Symbol: Flt3lg
• Standard Curve:
  
  Rat Flt-3 ligand ELISA Kit Standard Curve