Mouse Itgb1 ELISA KIT (1 x 96 wells)

CAT#: EA102889

The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Fibronectin/FN1 with <15pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Fibronectin/FN1 tissue specificity: Isoform 2 is expressed in skeletal and cardiac muscles only (at protein level). Isoform 1 is very weakly expressed in striated muscles and not The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detected in adult skeletal muscle fibers and cardiomyocytes. .

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Fibronectin
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of Mouse Fibronectin
• Sensitivity: <15pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 10ng/tube
• Background: Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
• Gene Symbol: Itgb1
• Standard Curve:
  
  Mouse Fibronectin ELISA Kit standard curve