Human Anti-SARS-CoV-2 (N Protein) IgG ELISA Kit (1x96 Wells)

CAT#: EA200001

Human Anti-SARS-CoV-2 (N Protein) IgG ELISA Kit (1x96 Wells)

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Specifications

Product Data

• Assay Type: Indirect
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Specificity: This kit is used for the detection of SARS-CoV-2 nucleocapsid protein human IgG antibodies
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • SARS-CoV-2 N Protein ELISA Plate (Alternating (+) and (-) Strips)|1 plate
  • Positive Control (Anti-N murine monoclonal antibody)|0.5 mL
  • Negative Control|0.5 mL
  • Sample Diluent|30 mL
  • Peroxidase Conjugate|12 mL
  • Wash Buffer Concentrate 20X|60 mL
  • ABTS Peroxidase Substrate|12 mL
  • Stop Solution|10 mL
• Principle: Microtitration wells coated with alternating strips of recombinant SARS-CoV-2 nucleocapsid protein (N) viral antigen and negative control antigen are exposed to test specimens, which may contain human IgG antibodies directed against SARS-CoV-2 N protein. After incubation period, unbound antibodies in the test sample are washed away. Bound SARS-CoV-2 N protein IgG antibodies are then incubated with an anti-human/mouse IgG peroxidase conjugate. Following a second wash cycle, the bound peroxidase conjugate is detected by the addition of ABTS peroxidase substrate. The assay is measured spectrophotometrically to indicate the abundance of SARS-CoV-2 N protein human IgG antibodies presented in a sample.
• Incubation Time: 30 mins
• Background: COVID-19 is a symptom after contracting SARS-CoV-2. SARS-CoV-2 is a single strand RNA coronavirus. It is composed of several proteins including Spike (S) protein, Envelope (E) protein, membrane (M) protein, and nucleocapsid (N). Research data show that the RBD region of the spike protein could bind to ACE2 receptor on the human cell membrane and use it as a mechanism for cell entry. IgG is the most abundant immunoglobulin to be found and produced in human body in response to an invader. Specific IgG is produced 7-14 days after contracting SARS-CoV-2.
• Gene Symbol: N
• Gene ID: 43740575
• Note: Review complete instructions before performing the test. 2. Strips of ELISA plate are removable. Remove unused strips and store as described under "Storage and Stability". Before testing begins, the user should inspect ELISA strip holders and ensure all strips are secure. A white stabilizer residue is normally observed on the bottom of unused wells.