Human REG1 alpha (REG1A) ELISA KIT (1 x 96 wells)

CAT#: EA200007

Human REG1 alpha (REG1A) ELISA KIT (1 x 96 wells)

Product Images

Data image for REG1 alpha (REG1A) ELISA Kit.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Human serum, plasma and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of Human PSP/REG1A
• Sensitivity: 5.3pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • PSP/REG1A Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant Human PSP/REG1A Standard (50ng/ml)|0.1 mL
  • 100x Biotinylated Human PSP/REG1A Detection Antibody|0.12 mL
  • 100x Peroxidase Conjugate|0.12 mL
  • Assay Buffer|30 mL
  • Sample Diluent|15 mL
  • Wash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
• Background: Pancreatic stone protein (PSP), also known as lithostathine, and regenerating protein (REG1A). It is a secreted, type I subclass member of the regenerating protein family. Mature human PSP/REG1A shares 76% and 69% aa sequence identity with mouse and rat PSP/REG1A, respectively. PSP/REG1A is constitutively expressed by pancreatic acinar cells, and is also induced in proliferating cell types such as colonic epithelium, islet beta-cells, and multiple tumor types. The 16 kDa precursor of PSP/REG1A has been observed mainly in the pancreas and is transiently elevated in acute and chronic pancreatitis. PSP/REG1A has been found both in blood and pancreatic secretions. PSP/REG1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Under healthy conditions PSP/REG1A is expressed at low levels in the pancreas. Upon focal or systemic inflammation, PSP/REG1A is strongly increased. PSP/REG1A is elevated in various conditions such as sepsis, ventilator associated pneumonia (VAP), chronic obstructive pulmonary disease (COPD) exacerbation, and type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). These observations indicated that PSP/REG1A might respond to infectious conditions and organ failure. The use of the PSP/REG1A as a biomarker has been confirmed the high diagnostic accuracy for sepsis. In addition, evidences obtained regarding PSP/REG1A and its isoforms on regeneration, growth and mitogenesis in gastrointestinal neoplastic diseases suggest that PSP/REG1A can be used as biomarker in the prediction of prognosis for neoplastic diseases.
• Gene Symbol: REG1A
• Gene ID: 5967
• Standard Curve:
  
  Data image for REG1 alpha (REG1A) ELISA Kit.