Human S100 beta (S100B) ELISA Kit 1 x 96

CAT#: EA200016

Human S100 beta (S100B) ELISA Kit 1 x 96

Product Images

Data image for Hu S100B ELISA kit.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 0.5 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 78pg/ml-5000pg/ml
• Sample Type: Human serum, plasma and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of Human S100B
• Sensitivity: 23pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • S100B Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant Human S100B Standard (250ng/ml)|0.1 mL
  • 100x HRP conjugated S100B Detection Antibody|0.12 mL
  • Assay Buffer|20 mL
  • Sample Diluent 120 mL Sample Diluent 220 mLWash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
  • Plate Sealer2 pieces
• Background: S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. S100B is glial-specific and is expressed primarily by astrocytes. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer disease, Down syndrome, epilepsy, amyotrophic lateral sclerosis, schwannoma, melanoma, and type I diabetes mellitus. S100B is secreted by astrocytes or can spill from injured cells and enter the extracellular space or bloodstream. Serum levels of S100B increase in patients during the acute phase of brain damage. S100B has emerged as a candidate peripheral biomarker of blood–brain barrier (BBB) permeability and CNS injury. In addition, S100B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
• Gene Symbol: S100B
• Gene ID: 6285
• Standard Curve:
  
  Data image for Hu S100B ELISA kit.