Human Serum Amyloid A (SAA1) ELISA Kit 1 x 96

CAT#: EA200018

Human Serum Amyloid A (SAA1) ELISA Kit 1 x 96

Datasheet
SDS

USD 582.00

In Stock*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich
Assay Length 4.5 hours incubations; 0.5 hour washing and analyzing samples
Signal Colorimetric
Curve Range 3.125ng/ml-200ng/ml
Sample Type Human serum, plasma and other biological fluids.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of Human SAA1
Sensitivity 0.3ng/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • SAA1 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant Human SAA1 Standard (10µg/ml)|0.1 mL
  • 100x Biotin conjugated SAA1 Detection Antibody|0.12 mL
  • 100x Streptavidin-HRP|0.12 mL
  • Detection Antibody Diluent|12 mL
  • Assay Buffer|12 mLSample Diluent30 mL
  • Wash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
  • Plate Sealer3 pieces
Background Serum amyloid A1 (SAA1) is a major acute-phase protein mainly produced by hepatocytes in response to infection, tissue injury and malignancy. When released into blood circulation, SAA1 is present as an apolipoprotein associated with high-density lipoprotein (HDL). SAA1 is a major precursor of amyloid A (AA), the deposit of which leads to inflammatory amyloidosis. Mature SAA1 consists of 104 amino acids with an apparent molecular weight of 12,500. Native SAA1 is a hexamer with each subunit assuming an antiparallel 4-helix bundle structure. Inflammatory cytokines such as IL-1β, IL-6 and TNF-α are major stimulants for hepatocyte expression of the SAA1 gene. SAA1 has been a clinical indicator and reliable biomarker for inflammatory diseases, chronic metabolic disorders and late-stage malignancy.
Gene Symbol SAA1
Gene ID 6288
Standard Curve
Serum Amyloid A (SAA1) ELISA Kit data image.

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