SARS-CoV-2 SARS-COV-2N ELISA Kit 1 x 96

CAT#: EA200029

SARS-CoV-2 SARS-COV-2N ELISA Kit 1 x 96

Product Images

Data image of SARS-CoV-2 SARS-COV-2N ELISA Kit.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 0.5 hour washing and analyzing samples
• Signal: Colorimetric
• Sample Type: Human serum, plasma and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of SARS-COV-2N
• Sensitivity: 3.4pg/ml
• Reactivities: SARS-COV-2N
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • SARS-COV-2N Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant SARS-COV-2N Standard (25ng/ml)|0.1 mL
  • 100x Biotin conjugated SARS-COV-2N Detection Antibody|0.12 mL
  • 100x SA-HRP Conjugate0.12 mL
  • Assay Buffer|60 mL
  • Wash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
  • Plate Sealer|3 pieces
• Background: Severe acute respiratory syndrome coronavirus 2 nucleocapsid (SARS-CoV-2 N) protein is an abundant RNA-binding protein critical for viral genome packaging. SARS-CoV-2 is an enveloped, positive-strand RNA virus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the COVID-19 pandemic. The nucleocapsid (N) protein of SARS COV-2 is 419 residues long, which is the most highly expressed of the four major coronavirus structural proteins. In addition to its interactions with RNA, the N protein forms protein-protein interactions with the coronavirus membrane protein (M) during the process of viral assembly. It also has additional functions in manipulating the cell cycle of the host cell. The N protein is highly immunogenic and antibodies to N protein are found in patients recovered from Covid-19. The N protein is composed of two main protein domains connected by an intrinsically disordered region (IDR) known as the linker region, with additional disordered segments at each terminus. Both the N-terminal and C-terminal domains are capable of binding RNA. The C-terminal domain forms a dimer that is likely to be the native functional state.
• Standard Curve:
  
  Data image of SARS-CoV-2 SARS-COV-2N ELISA Kit.