Human IGF1 ELISA KIT (96 T)

CAT#: EA800086

Human IGF-1 Immunoassay

Datasheet
Size
    • 96 T

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Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 31.25-2000pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant human IGF-1.
Sensitivity 15pg/mL
Reactivities Human
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
Background Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity. The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
Gene Symbol IGF1

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