ATIC (NM_004044) Human 3' UTR Clone

CAT#: SC201041

3`UTR clone of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATIC
• Synonyms: AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH
• ACCN: NM_004044
• Insert Size: 147 bp
• Sequence Data:
  Insert Sequence

  >SC201041 3'UTR clone of NM_004044
  The sequence shown below is from the reference sequence of NM_004044. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCGCTCATACGAACCTTCGGCTCTTCCACCACTGATTTTACCACACACTGTTTTTTGGCTTGCTTATGT
  GTAGGTGAACAGTCACGCCTGAAACTTTGAGGATAACTTTTTAAAAAAATAAAACAGTATCTCTTAATCA
  CTGGATC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004044.6
• Summary: 'This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009]'
• Locus ID: 471