SF3B14 (SF3B6) (NM_016047) Human 3' UTR Clone

CAT#: SC202065

3`UTR clone of splicing factor 3B 14 kDa subunit (SF3B14) for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol SF3B6
Synonyms CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
ACCN NM_016047
Insert Size 204
Sequence Data
>SC202065 3'UTR clone of NM_016047
The sequence shown below is from the reference sequence of NM_016047. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

GGCATCAACACAGATCCACCAAAATAAATGTTTTCTACATTTTCATTTGGACTAAATCCCACGAATGACA
ACTACCACCTTTTTTTCCTTTTTAATTAATACTAAATATTGTGATTTCTTATTTGAGGTTCAAAATGACC
TGCTTGAAACTTTGATACATATTGGAATACATTATGTTAATAAACTTGTAGCTTTTTGTGAAAC

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_016047.3
Summary This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
Locus ID 51639

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