Apc2 (ANAPC2) (NM_013366) Human 3' UTR Clone

CAT#: SC202077

3`UTR clone of anaphase promoting complex subunit 2 (ANAPC2) for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol ANAPC2
Synonyms APC2
ACCN NM_013366
Insert Size 181
Sequence Data
>SC202077 3'UTR clone of NM_013366
The sequence shown below is from the reference sequence of NM_013366. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TCTACCGCCTGCCCAAGAACTGCAGCTGACACATCGCCCGCCCGCCCGCCCGCCCGCCAGGCGCTGCCCT
GCAGGTGCTCTCGTCCTCCCGTGCCAGCCCCCGCCCGCCCGTGTCCCAGAATGCACTGCTGAGGAGCATG
CCCACCCCCACCCCCGCAGTGTGCAGATTAAAGCAAGTCAG

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_013366.3
Summary A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]
Locus ID 29882

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