ALG3 (NM_001006941) Human 3' UTR Clone

CAT#: SC202275

3`UTR clone of asparagine-linked glycosylation 3 alpha-13- mannosyltransferase homolog (S. cerevisiae) (ALG3) transcript variant 4 for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol ALG3
Synonyms CDG1D; CDGS4; CDGS6; D16Ertd36e; not; Not56; NOT56L
ACCN NM_001006941
Insert Size 189
Sequence Data
>SC202275 3'UTR clone of NM_001006941
The sequence shown below is from the reference sequence of NM_001006941. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

CAACACAGCAAGAAAGCCCACTGAAGTCCACCCCTTTCCCTCAGGACCTGAGTCTACCCTCAGGACCTGG
GGTTGGTTGGACTCTGCCCTTCCAAATAAACCTTGCTAAGTCCAACTCTGTGCAACCTACATGGAGGTGG
GGGCAGCCGATGCCTGGTCCAGGCTGTGAGGGACACGTATGGAGCAGAT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_001006941.2
Summary This gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosylation type Id (CDG-Id) characterized by abnormal N-glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
Locus ID 10195

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