Ribonuclease A (RNASE1) (NM_002933) Human 3' UTR Clone
CAT#: SC202974
3`UTR clone of ribonuclease RNase A family 1 (pancreatic) (RNASE1) transcript variant 4 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | RNASE1 |
Synonyms | RAC1; RIB1; RNS1 |
ACCN | NM_002933 |
Insert Size | 268 bp |
Sequence Data |
>SC202974 3'UTR clone of NM_002933
The sequence shown below is from the reference sequence of NM_002933. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC GATGCTTCTGTGGAGGACTCTACCTAAGGTCAGAGCAGCGAGATACCCCACCTCCCTCAACCTCATCCTC TCCACAGCTGCCTCTTCCCTCTTCCTTCCCTGCTGTGAAAGAAGTAACTACAGTTAGGGCTCCTATTCAA CACACACATGCTTCCCTTTCCTGAGTCCCATCCCTGCGTGATTTTGGGGGTGAAGAGTGGGTTGTGAGGT GGGCCCCATGTTAACCCCTCCACTCTTTCTTTCAATAAAACGCAGTTGCAAACACCTG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_002933.4 |
Summary | 'This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]' |
Locus ID | 6035 |
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