SARDH (NM_001134707) Human 3' UTR Clone

CAT#: SC204573

3`UTR clone of sarcosine dehydrogenase (SARDH) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SARDH
• Synonyms: BPR-2; DMGDHL1; SAR; SARD; SDH
• ACCN: NM_001134707
• Insert Size: 339 bp
• Sequence Data:
  Insert Sequence

  >SC204573 3'UTR clone of NM_001134707
  The sequence shown below is from the reference sequence of NM_001134707. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAACAAGAGGGTGAAGGGAATCTACTGAGGGCTCAGACCACATGCCCCATCCCATGCTGTCCATCCATTC
  TCACAGGTCCCAAATGGCCCTGGGTCCCAGGACCCTGGACCCAGCCAGAACTTGTGCCCTCCCTGTCCTT
  AGCCTAGGCCTAGTCCTGCGAAAACCCCCAAACCAGATACGGACAGAGACCAAGATGGCCCATCCTGTGG
  TCACTGACTGGAAGGCAGTCTCTAATCCACCCCAATCCTCCCAGAAAGCAAACCTGTAAGTGTCTTGGGC
  TCAGGGACCATGGCTTGTAGCCCTTTCCAAGCTTCTAGAAGGTGCTTAGTAAATACTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001134707.1
• Summary: 'This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008]'
• Locus ID: 1757