ITPKA (NM_002220) Human 3' UTR Clone

CAT#: SC205174

3`UTR clone of inositol 145-trisphosphate 3-kinase A (ITPKA) for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol ITPKA
Synonyms IP3-3KA; IP3KA
ACCN NM_002220
Insert Size 400 bp
Sequence Data
>SC205174 3'UTR clone of NM_002220
The sequence shown below is from the reference sequence of NM_002220. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

GGACAATCTCATTGGCATCCTGGCCAGCCTGGCTGAGAGATGAGGCTGGACTCCTGTCCCCGCGGGCCGC
TCACCTGACATGTGGACCTGCAGCTTTGTCCCCACTGTGCATGCCGGCTTGAGACTGGAGCCCCGCGGTG
CAGGGCAGTTCACCGGGTCCTGCAGGACCAGGTGCCAGCCACTAAGGGGGGGCACCGCCGATGCCAGGGG
TTTTGCCCACCCGGGCCCCAGCGTTCCCAGAGCCAAATGACACTAACTTATAGAAGGGGAGGGGGCAAAG
GGCTTCTTCCTCAGGCCAGCTCTTCTGAGGAGGCTCTGCCCTCTCCAGAGGTGCCAGACCGCGGATTTTA
TTTAGCAAGCCCAGACCTTCCGGTCTAACGTCTCACACCACGACGGACTC

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_002220.1
Summary 'Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.[provided by RefSeq, Apr 2011]'
Locus ID 3706

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.