PPCS (NM_001077447) Human 3' UTR Clone

CAT#: SC206600

3`UTR clone of phosphopantothenoylcysteine synthetase (PPCS) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPCS
• Synonyms: RP11-163G10.1
• ACCN: NM_001077447
• Insert Size: 530
• Sequence Data:
  Insert Sequence

  >SC206600 3'UTR clone of NM_001077447
  The sequence shown below is from the reference sequence of NM_001077447. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTCAGTCTCGACACACAGCTTTTATAGGTGACAGAAACTGAAGTAAAAAGCCCTTATAGGATCAAAAAT
  TGTTCAGGGCTCTTAGAGATGGTGAAAACTACAAAAAAAACCATGGCTTTCATATGGACAGATAAAATGA
  AAGAAAGGGAAAAGGCAGTGGTGTGTAGGCAAATATGGTTTGGCATTTGTCTTTTAATGACACCTGATAT
  GATGTCATTTTGATTTTGAAATTGAACACTAGAACTGTTAATCACCTTTAAAAAGAAGAGCTTATTGGGA
  ATTATATATTCCTTAAAATATACATGGGGGCCTGAATGTCAGCCATCTTTATACTATAGAAAAAGGATTA
  TGGATGCATGAATGGTCATGCTTTGGAGATCAAATATTGGTTGAATGCCTATGTATGTCAGGCCCTGTGC
  TGAGCCATGAGGATTAAAAAGATGAATAAACATATCTTGTTTAGGAAATGGATGTATAAAAAAATCAAGT
  GCAATAAAGTGTGTGTCCAAAAGCTGACACAATGGAAAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001077447.1
• Summary: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM, Mar 2008]
• Locus ID: 79717