VPS25 (NM_032353) Human 3' UTR Clone

CAT#: SC207378

3`UTR clone of vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VPS25
• Synonyms: DERP9; EAP20; FAP20
• ACCN: NM_032353
• Insert Size: 557
• Sequence Data:
  Insert Sequence

  >SC207378 3'UTR clone of NM_032353
  The sequence shown below is from the reference sequence of NM_032353. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCGAGATCATCACTGTCAGCGATGGCCGAGGCGTCAAGTTCTTCTAGCAGGGACCTGTCTCCCTTTACTT
  CTTACCTCCCACCTTTCCAGGGCTTTCAAAAGGAGACAGACCCAGTGTCCCCCAAAGACTGGATCTGTGA
  CTCCACCAGACTCAAAAGGACTCCAGTCCTGAAGGCTGGGACCTGGGGATGGGTTTCTCACACCCCATAT
  GTCTGTCCCTTGGATAGGGTGAGGCTGAAGCACCAGGGAGAAAATATGTGCTTCTTCTCGCCCTACCTCC
  TTTCCCATCCTAGACTGTCCTTGAGCCAGGGTCTGTAAACCTGACACTTTATATGTGTTCACACATGTAA
  GTACATACACACATGCGCCTGCAGCACATGCTTCTGTCTCCTCCTCCTCCCACCCCTTTAGCTGCTGTTG
  CCTCCCTTCTCAGGCTGGTGCTGGATCCTTCCTAGGGGATGGGGGAAGCCCTGGCTGCAGGCAGCCTTCC
  AGGCAATATGAAGATAGGAGGCCCACGGGCCTGGCAGTGAGAGGTGTGGCCCCACACCGATTTATGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_032353.2
• Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]
• Locus ID: 84313