LIAS (NM_006859) Human 3' UTR Clone

CAT#: SC207379

3`UTR clone of lipoic acid synthetase (LIAS) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol LIAS
Synonyms HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD
ACCN NM_006859
Insert Size 575
Sequence Data
>SC207379 3'UTR clone of NM_006859
The sequence shown below is from the reference sequence of NM_006859. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TTTCCTGAAAAATCTAGTGGCTAAAAGAAAAACAAAAGACCTCTAAAACTTCAACAAGACCTTCAAGATC
ACAGAAATTTTTAAAATTTGATTCCAGTTAATAACAGAGGTGGTGCCAGAATGCCTGGACTGCAGTGGAT
GTGCCCCACCTCTTTGCTTAAAAAAAAAAATGTCAATAGCCAGGCATAGTGGCTCACGCCTGTAATCCCA
GCACTTTAGGAGGCCAAGGCGGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGT
GAAATCCTGTCTCCACTAAAAACACAAAAATTAGTCAGGCGTGGTAGTGGGTGCCTGTAATCCCAGCTAC
TCGGGAGGCTAAGGCAGGAGAATCACTTGAACCTGGGAGGGGGAGGTTGCAGTGAGCCAAGATCGCTCCA
TTGCCCTCCAGCCTGGGTGACAAGAGCAAAACTCCATCTCAAATAAATAAAAAGGAAAAAAAAGTCAATA
AACTGTACAAATTTAATTACAGTAATTCATTTGGCTTTTAGTGTATCTTCTTTGTCCCAATTAAACAGTT
GCTTGCTGTGATGTA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_006859.2
Summary The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Locus ID 11019

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