RPB11 (POLR2J) (NM_006234) Human 3' UTR Clone

CAT#: SC207539

3`UTR clone of polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa (POLR2J) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR2J
• Synonyms: hRPB14; POLR2J1; RPB11; RPB11A; RPB11m
• ACCN: NM_006234
• Insert Size: 562 bp
• Sequence Data:
  Insert Sequence

  >SC207539 3'UTR clone of NM_006234
  The sequence shown below is from the reference sequence of NM_006234. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCATAAAAGACAAGCAGGAAGGAATTGAGTAGGGGCCAGAGGGGGCTCTGCTCGGCCTGTGAGCCCCGT
  TCCTACCTGTGCCTGACCCTCCGCTCCAGGTACCACACCGAGGAGAGCGGCCGGTCCCAGCCATGGCCCG
  CCTTGTGGCCACCCCTCACCCTGACACCGACGTGTCCTGTACATAGATTAGGTTTTATATTCCTAATAAA
  GTATAGCGGGAGAGACCTGGATGTGGACTTGAGCAGCGGTGACTTCGCAAGCAAATGGATTGTCAGGCTT
  GATGCAGGCAGATGACCTGTTTCAGGGGCGTCCGGCTGGCAGGGATGAATTCATTCTGGACCAAAGATCC
  GGGGTCCAGGGGCTGCTGCGGGGGCTGTGCTGAGCCGGAGAGAAGTGTGCAAACCCATGAGCTCCCAAGA
  GTCTCTGCTCTAGAAGCCTCAACTCCTGGGCCTGCCTGTCAGTCAAAGCAGGAACACTTCTTCCTGCATA
  ACTCGAAACACCTTTCCACAGGCTTCTTGTCCACAGTAGAGTTTAATAAAAATATTCACTGAAAGACCCC
  CC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006234.4
• Summary: 'This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]'
• Locus ID: 5439