LIAS (NM_194451) Human 3' UTR Clone

CAT#: SC207818

3`UTR clone of lipoic acid synthetase (LIAS) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol LIAS
Synonyms HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD
ACCN NM_194451
Insert Size 611
Sequence Data
>SC207818 3'UTR clone of NM_194451
The sequence shown below is from the reference sequence of NM_194451. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

ATATGCAGCCAACAAGGCGTCACCTTAAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAAAAGAAAAACA
AAAGACCTCTAAAACTTCAACAAGACCTTCAAGATCACAGAAATTTTTAAAATTTGATTCCAGTTAATAA
CAGAGGTGGTGCCAGAATGCCTGGACTGCAGTGGATGTGCCCCACCTCTTTGCTTAAAAAAAAAAATGTC
AATAGCCAGGCATAGTGGCTCACGCCTGTAATCCCAGCACTTTAGGAGGCCAAGGCGGGTGGATCACCTG
AGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAATCCTGTCTCCACTAAAAACACAAAAATTAG
TCAGGCGTGGTAGTGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTAAGGCAGGAGAATCACTTGAACCT
GGGAGGGGGAGGTTGCAGTGAGCCAAGATCGCTCCATTGCCCTCCAGCCTGGGTGACAAGAGCAAAACTC
CATCTCAAATAAATAAAAAGGAAAAAAAAGTCAATAAACTGTACAAATTTAATTACAGTAATTCATTTGG
CTTTTAGTGTATCTTCTTTGTCCCAATTAAACAGTTGCTTGCTGTGATGTA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_194451.1
Summary The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Locus ID 11019

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