NLRP3 (NM_001079821) Human 3' UTR Clone

CAT#: SC208108

3`UTR clone of NLR family pyrin domain containing 3 (NLRP3) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NLRP3
• Synonyms: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1
• ACCN: NM_001079821
• Insert Size: 593
• Sequence Data:
  Insert Sequence

  >SC208108 3'UTR clone of NM_001079821
  The sequence shown below is from the reference sequence of NM_001079821. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTTGAGCCTTCTTGGTAGGAGTGGAAACGGGGCTGCCAGACGCCAGTGTTCTCCGGTCCCTCCAGCTGG
  GGGCCCTCAGGTGGAGAGAGCTGCGATCCATCCAGGCCAAGACCACAGCTCTGTGATCCTTCCGGTGGAG
  TGTCGGAGAAGAGAGCTTGCCGACGATGCCTTCCTGTGCAGAGCTTGGGCATCTCCTTTACGCCAGGGTG
  AGGAAGACACCAGGACAATGACAGCATCGGGTGTTGTTGTCATCACAGCGCCTCAGTTAGAGGATGTTCC
  TCTTGGTGACCTCATGTAATTAGCTCATTCAATAAAGCACTTTCTTTATTTTTCTCTTCTCTGTCTAACT
  TTCTTTTTCCTATCTTTTTTCTTCTTTGTTCTGTTTACTTTTGCTCATATCATCATTCCCGCTATCTTTC
  TATTAACTGACCATAACACAGAACTAGTTGACTATATATTATGTTGAAATTTTATGGCAGCTATTTATTT
  ATTTAAATTTTTTGTAACAGTTTTGTTTTCTAATAAGAAAAATCCATGCTTTTTGTAGCTGGTTGAAAAT
  TCAGGAATATGTAAAACTTTTTGGTATTTAATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001079821.2
• Summary: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]
• Locus ID: 114548