eNOS (NOS3) (NM_001160111) Human 3' UTR Clone

CAT#: SC208555

3`UTR clone of nitric oxide synthase 3 (endothelial cell) (NOS3) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NOS3
• Synonyms: ECNOS; eNOS
• ACCN: NM_001160111
• Insert Size: 653 bp
• Sequence Data:
  Insert Sequence

  >SC208555 3'UTR clone of NM_001160111
  The sequence shown below is from the reference sequence of NM_001160111. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TAGTTGGGACTACAGGCGCATGCCATGATGCCTAGCTAATTTTTGTATTTTTTATAGAGATGGGGTTTCG
  CCATGTTGCCCAGGCTGGTCTCTAACTCCTGGGTTCAAGCAATCCACCTGCCTCGGCCTCCCAAAGTGCT
  GCGATTATAGACGTGAGCCACTGCACCTGGCCCTCAGTATCTTAAGCAAGTTGGAATCTCGTGAAACCCT
  TTTTGCTGCCTTAGTGTCCGTTTCAGCCCTCATTCTGACCTACCTTTTCAAGAAAAATAGCACCAGCAAT
  TGACTTTTTTTTAGCATAAAGGTGTATAGACACCCATATAACCTACAGCCTTCACAAGGCATAGCACATT
  TTCACCACCCTGGAAAGTTCCCTCATCAGTTCCTCACGTGAATCCCTTCCCAGTCTGTCTCCCTGCCAGA
  AGTGTCTGTCACCACAGAATAGTTTCGCCTGCTCTAGAACGGCACCTAGATGGAAGCACGCAGTGTTGCG
  GCGTCTCCTGCTGAGGCTGTTTTTGAGGCGCACTCGTGTTGCTGCGTGACTCAGTATTTCACTCATTCTG
  CTGCTGAGTGCCGTTCATTGTGTGAATATCCCCAGTTTGTTTACCCATTCTCTTGTTGGTGACACTTGGG
  CTGTTTCCAGGTCGGGGCTATTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001160111.1
• Summary: 'Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]'
• Locus ID: 4846