POLR3B (NM_018082) Human 3' UTR Clone

CAT#: SC208621

3`UTR clone of polymerase (RNA) III (DNA directed) polypeptide B (POLR3B) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR3B
• Synonyms: C128; HLD8; INMAP; RPC2
• ACCN: NM_018082
• Insert Size: 664
• Sequence Data:
  Insert Sequence

  >SC208621 3'UTR clone of NM_018082
  The sequence shown below is from the reference sequence of NM_018082. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGGTTAAAACTGTCCAAGTACAATGAATGAGGATGGAAAAAATGATTATTAAAGAGAACAAGTGATAC
  ATCCAATGCAACGGAAAGCAGAAGGGATTTAGGACTACGTCTCCTCCTGTGAAGAATTCCCTTGCGTATT
  CTCTCTCTAAAACAACCAAAAAAAAATGGAGAGGCTTTTTATATACTCTAAGACTGGCTAAACAACCTTG
  ATCATTGAGCCTCGAGCCATGGGAGAGATGCTGACCATGTGGACTGCAAGGCTGCTTGATTCACAGATGG
  ATGTGACCTAAAGGATAAATAAGCTATTACTTATGTGCTGATCTCTTGACATTCACTCATTAGAAGACCT
  TACTCCTTCAAGCAAATGTTTGGGGTCAAATTTACCATATCTTCTGGCTAACCATATTCAAGATTCTTCT
  GAAACTTGGAGGATGTAAAGAATCCATTTGATTTGGTCAGCCTGGCTTTTGTCGTGGTGGCTGGCTCGGA
  TAAATTTTCCCAACAATTAAATCTTGCCTTTACACACCCAAACTTTGTAATTTTAGTCTTGGTGAAATAT
  AATGAATTTGTTCCTACCTTGTCAAGCAAGAATGTCGTCTTCTCCTATGGACTCAATTGCTATTATTTTA
  AACCTGCATGATTGTACCATGCAATACTATTCGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_018082.5
• Summary: This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
• Locus ID: 55703