MLKL (NM_152649) Human 3' UTR Clone

CAT#: SC208650

3`UTR clone of mixed lineage kinase domain-like (MLKL) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MLKL
• Synonyms: hMLKL
• ACCN: NM_152649
• Insert Size: 644
• Sequence Data:
  Insert Sequence

  >SC208650 3'UTR clone of NM_152649
  The sequence shown below is from the reference sequence of NM_152649. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAACTCTCCACCTTTTCTAAGTAGTGTATCAAAATCTAAACCAAGGAGTCTCTGGACAAGAAGCTGGGAG
  AGGCACAAACTGGACATCTCTCTCTCTCATATCCTTCGGCATTGGGTTATCTATGGGTGCAAGGAGTGGG
  CACGCTTCTCTGTTACAAATAGAAAACGATTCCAGTCATACAGGACACATCCCACTCCAAATGATATTTC
  CAAAAACATACCTCTGACAGTAACTTTGATAGATGGTTTGTCAAATGTATCTTTCTGGGTATCCACACCT
  CTTGGCAATGAAATTTGCAGCTCCTCCCTTCCATAAATGAAGTCTCTTTCCCCACCATTTGAATCTGGGC
  TGGCACTGTGACTTGATTTGATCAATAGAATGTGGAAGAAGTGACTGTATGCCAGTTCCAAGCCTAGGTT
  TCAAGAGGCCTTATAAATGTCTGTTGGAACCTTACCCAGCCATGAACATGTTGAGTGAGCATGCTGGAGA
  ATGAGAGACCACATGAAGCAGAAACATGCTTTCCTAGCTGAAGTCATACTAGCCCAACCAACATGGCAGC
  TAACACATGAATGAGGCCAATCAAGACCAGAAGAACCACTCAAGCAGATCCCAGCCCAAATTGCCCATTC
  ACACAATCAGGAGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_152649.2
• Summary: This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
• Locus ID: 197259